壳聚糖对无氧训练大鼠肝脏自由基代谢的影响

doi:10.3969/j.issn.2095-4344.2012.51.014

摘要/Abstract

摘要：

背景：壳聚糖具有清除自由基的功能。
目的：观察补充壳聚糖对以糖酵解供能为主间歇性周期游泳训练大鼠肝脏功能的影响。
方法：将64只成年SD大鼠随机分为对照组、训练组、服药组、服药训练组，每组16只。训练组与服药训练组进行以糖酵解供能为主的间歇性周期训练，于训练第12天两组随机取8只大鼠进行相对大运动量游泳运动(即定量负荷运动)，对照组与服药组未进行间歇性周期训练，但也于相同时间点进行定量负荷运动，即各组均分定量负荷前与定量负荷运动后两亚组。服药训练组每次训练前30 min灌服壳聚糖0.3 g/kg，服药组于相同时间点灌服壳聚糖0.3 g/kg，对照组与训练组灌以等量生理盐水。
结果与结论：与定量负荷运动前比较，各组定量负荷运动后超氧化物歧化酶活性明显降低(P < 0.01)，丙二醛水平明显升高(P < 0.01)。且各组定量负荷运动后超氧化物歧化酶活性均明显低于定量负荷运动前对照组(P < 0.01)。与定量负荷运动后对照组比较，定量负荷运动后训练及服药训练组超氧化物歧化酶活性明显升高(P < 0.01)，丙二醛水平明显降低(P < 0.01)。与定量负荷运动后训练组比较，定量负荷运动后服药组超氧化物歧化酶活性降低(P < 0.05)，丙二醛水平升高(P < 0.05)；定量负荷运动后训练组超氧化物歧化酶活性升高(P < 0.05)，丙二醛水平降低(P < 0.05)。表明服用壳聚糖配合运动训练可有效提高机体抗氧化能力，改善自由基代谢。

Abstract:

BACKGROUND: Chitosan has the function of scavenging the free radicals.
OBJECTIVE: To observe the effect of chitosan on the liver function of rats undergoing glycolysis energy supply following intermittent cycle swimming training.
METHODS: Sixty-four adult Sprague Dawley rats were randomly divided into control group, exercise group, medication group, medication and exercise group, 16 rats in each group. The exercise group and the medication and exercise group performed to glycolysis mainly intermittent cycle training, and after training for 12 days, eight rats were selected randomly in two groups to take the relatively large amount of swimming exercise (i.e., a quantitative load exercise); the rats in the control group and medication group did not underwent the intermittent cycle training, but the rats in the two groups also received the quantitative load exercise at the same time points, i.e. each group was divided into two subgroups of pre-quantitative load exercise group and post-quantitative load exercise group. Rats in the medication and exercise group were gavaged with chitosan at the concentration of 0.3 g/kg at 30 minutes before training; rats in the medication group were gavaged with chitosan at the concentration of 0.3 g/kg at the same time point; rats in the control group and the exercise group were gavaged with the same amount of normal saline.
RESULTS AND CONCLUSION: The superoxide dismutase activity in each group after the quantitative load exercise was decreased significantly when compared with that before quantitative load exercise (P < 0.01), and the malondialdehyde level in each group was significantly increased (P < 0.01). And the superoxide dismutase activity in each group after the quantitative load exercise was significantly lower than that in the control group before quantitative load exercise (P < 0.01). Compared with the control group after quantitative load exercise, the superoxide dismutase activity in the exercise group and the medication and exercise group were increased significantly (P < 0.01), and the malondialdehyde levels were significantly reduced (P < 0.01). Compared with the exercise group after quantitative load exercise, the superoxide dismutase activity in the medication group after quantitative load exercise was decreased significantly (P < 0.05), and the malondialdehyde level was increased (P < 0.05); the superoxide dismutase activity inthe exercise group after the quantitative load exercise was increased (P < 0.05), and the malondialdehyde level was decreased (P < 0.05). It shows that chitosan combined with exercise and training can effectively improve the antioxidant ability and the metabolism of free radicals.

石颖. 壳聚糖对无氧训练大鼠肝脏自由基代谢的影响[J]. 中国组织工程研究, 2012, 16(51): 9574-9578.

Shi Ying. Influence of chitosan on liver free radical metabolism in anaerobic training rats[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(51): 9574-9578.

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本实验结果显示定量负荷游泳运动前，对照组、训练组、服药组肝脏丙二醛水平、超氧化物歧化酶活性基本一致，组间比较差异无显著性意义。服药组定量负荷游泳运动后丙二醛水平显著升高，超氧化物歧化酶活性显著降低。本文推测，定量负荷游泳运动前训练组经过1d的休息后，丙二醛、超氧化物歧化酶基本恢复到了安静时的水平，但由于本实验未对大鼠运动训练后的恢复情况进行动态观察，所以关于丙二醛、超氧化物歧化酶的恢复情况还有待于进一步验证；而定量负荷游泳运动前服药组单纯服用壳聚糖后，丙二醛水平、超氧化物歧化酶活性变化不明显，说明服用壳聚糖对大鼠安静状态时肝脏自由基代谢并无显著性影响。在定量负荷运动后即刻，服药组与对照组相比差异无显著性意义（P>0.05）。已有研究表明，壳聚糖含有羟基、氨基等机性基团，具有碱性，即刻吸附组织中的氢离子，改善内环境，又可选择性的吸附许多阴离子（如氧自由基、胆汁酸、色素、癌毒素等），排除人体毒素。本实验认为壳聚糖含有碱性极性基团，可直接吸附组织中的氧自由基，而不是通过提高机体抗氧化酶的活性来清除自由基。结果表明，安静状态肝脏脂质过氧化反应程度小，显示不出服用壳聚糖的必要性。

定量负荷游泳运动后，服药组与训练组相比，丙二醛水平显著升高，超氧化物歧化酶活性显著降低。本实验认为由于连续7d的间歇性周期训练，提高了机体的氧化能力，同时也提高了机体的抗氧化能力，使得机体在运动过程中脂质过氧化的趋势得以和缓。结果表明，运动训练比单纯服用壳聚糖能够有效提高机体抗氧化能力，改善自由基代谢。